BADERC-Brian Seed

Brian Seed, Ph.D.

Genetic Analysis of Signal Transduction

Following on our earlier development of methods for expression cloning of cell surface receptors, we have automated a method to accelerate the identification of the intracellular paths that underly cellular communication and pathogenic responses in the immune system. As part of that program we have created robotic approaches that allow the rapid identification of genes that control transcriptional activation, subcellular localization and receptor deactivation. We have also developed systems for rapid generation of mice bearing targeted disruptions of specific candidate genes. We are integrating the two approaches with an emphasis on signaling pathways that influence organismic responses to metabolic load and pathogens.

References:

Jiang, C. Ting, A.T. and Seed, B. PPARg agonists inhibit production of monocyte inflammatory cytokines. Nature 1998; 391, 82-86.
Xavier, R., Brennan, T., Li, Q., McCormack, C., and Seed, B. Membrane compartmentation is required for efficient T cell activation. Immunity 1998; 8, 723-732.
Fukumura, D., Xavier, R., Sugiura, T., Chen, Y., Park, E.C., Lu, N., Selig, M., Nielsen, G., Taksir, T., Jain, R.K., and Seed, B. Tumor Induction of VEGF Promoter activity in stromal cells. Cell 1998; 94, 715-725.
Pickl, W.F., Pimentel, F.X.P., Seed, B. Lipid rafts and pseudotyping. J. Virol. 2000 75; 7175-83.
Randow, F., Seed B., Endoplasmic reticulum chaperone gp96 is required for innate immunity but not cell viability. Nat. Cell Biol. 2001; 10, 891-6.
Yang, Y., Seed, B. Site-specific gene targeting in mouse embryonic stem cells with intact bacterial artificial chromosomes. Nat Biotechnol. 2003; 21, 447-51.
Wang X, Seed B. Selection of oligonucleotide probes for protein coding sequences.Bioinformatics. 2003;19:796-802.
Lichtenthaler SF, Dominguez DI, Westmeyer GG, Reiss K, Haass C, Saftig P, De Strooper B, Seed B. The cell adhesion protein P-selectin glycoprotein ligand-1 is a substrate for the aspartyl protease BACE1. J Biol Chem. 2003;278:48713-9.
Medoff BD, Seed B, Jackobek R, Zora J, Yang Y, Luster AD, Xavier R. CARMA1 is critical for the development of allergic airway inflammation in a murine model of asthma. J Immunol. 2006 Jun 15;176(12):7272-7.
Ketteler R, Sun Z, Kovacs KF, He WW, Seed B. A pathway sensor for genome-wide screens of intracellular proteolytic cleavage. Genome Biol. 2008 Apr 3;9(4):R64. PMCID: PMC2643935.
Spandidos A, Wang X, Wang H, Dragnev S, Thurber T, Seed B. A comprehensive collection of experimentally validated primers for Polymerase Chain Reaction quantitation of murine transcript abundance. BMC Genomics. 2008 9:633 PMCID: PMC2631021
Adams JC, Seed B, Lu N, Landry A, Xavier RJ. Selective activation of nuclear factor kappa B in the cochlea by sensory and inflammatory stress. Neuroscience.
2009 160(2):530-9. PMCID: PMC2673023






			Brian Seed, Ph.D. Genetic Analysis of Signal Transduction Following on our earlier development of methods for expression cloning of cell surface receptors, we have automated a method to accelerate the identification of the intracellular paths that underly cellular communication and pathogenic responses in the immune system. As part of that program we have created robotic approaches that allow the rapid identification of genes that control transcriptional activation, subcellular localization and receptor deactivation. We have also developed systems for rapid generation of mice bearing targeted disruptions of specific candidate genes. We are integrating the two approaches with an emphasis on signaling pathways that influence organismic responses to metabolic load and pathogens. References: Jiang, C. Ting, A.T. and Seed, B. PPARg agonists inhibit production of monocyte inflammatory cytokines. Nature 1998; 391, 82-86. Xavier, R., Brennan, T., Li, Q., McCormack, C., and Seed, B. Membrane compartmentation is required for efficient T cell activation. Immunity 1998; 8, 723-732. Fukumura, D., Xavier, R., Sugiura, T., Chen, Y., Park, E.C., Lu, N., Selig, M., Nielsen, G., Taksir, T., Jain, R.K., and Seed, B. Tumor Induction of VEGF Promoter activity in stromal cells. Cell 1998; 94, 715-725. Pickl, W.F., Pimentel, F.X.P., Seed, B. Lipid rafts and pseudotyping. J. Virol. 2000 75; 7175-83. Randow, F., Seed B., Endoplasmic reticulum chaperone gp96 is required for innate immunity but not cell viability. Nat. Cell Biol. 2001; 10, 891-6. Yang, Y., Seed, B. Site-specific gene targeting in mouse embryonic stem cells with intact bacterial artificial chromosomes. Nat Biotechnol. 2003; 21, 447-51. Wang X, Seed B. Selection of oligonucleotide probes for protein coding sequences.Bioinformatics. 2003;19:796-802. Lichtenthaler SF, Dominguez DI, Westmeyer GG, Reiss K, Haass C, Saftig P, De Strooper B, Seed B. The cell adhesion protein P-selectin glycoprotein ligand-1 is a substrate for the aspartyl protease BACE1. J Biol Chem. 2003;278:48713-9. Medoff BD, Seed B, Jackobek R, Zora J, Yang Y, Luster AD, Xavier R. CARMA1 is critical for the development of allergic airway inflammation in a murine model of asthma. J Immunol. 2006 Jun 15;176(12):7272-7. Ketteler R, Sun Z, Kovacs KF, He WW, Seed B. A pathway sensor for genome-wide screens of intracellular proteolytic cleavage. Genome Biol. 2008 Apr 3;9(4):R64. PMCID: PMC2643935. Spandidos A, Wang X, Wang H, Dragnev S, Thurber T, Seed B. A comprehensive collection of experimentally validated primers for Polymerase Chain Reaction quantitation of murine transcript abundance. BMC Genomics. 2008 9:633 PMCID: PMC2631021 Adams JC, Seed B, Lu N, Landry A, Xavier RJ. Selective activation of nuclear factor kappa B in the cochlea by sensory and inflammatory stress. Neuroscience. 2009 160(2):530-9. PMCID: PMC2673023